Tourist Line Rules

Are there any requirements or regulations that you need to comply to become a tourist railroad? Do they have a lower or higher requirements than a short line freight road? Is there a maximum track quality needed? Are they allowed to be a short line that operates freight also as a daily occurence?

As tourist line you have to comply with same FRA rules as any railroad.
As for minimum track standard, anything above excepted track, but not many people like to ride on a 10 to 15 mph ride
Same licencing of Locomotive Engineers.

As for those rule :
http://www.access.gpo.gov/nara/cfr/wais ... v4_07.html Knock yourself out

Like Dutch said above, the same rules apply, especially when it comes to passenger service.

Landlocked operations (with no outside rail connections or public grade crossings) are sometimes given a little wiggle room (such as not requiring signals, etc), but otherwise the rules are the same.

Once state-regulated, locomotive boilers and pressure vessels are now federally regulated.

Some tourist lines might be part of non-profit museums, but if they also operate freight service for-profit, there may be some questions asked. Some freight lines lease trackage rights to tourist/museum lines to keep the operations separate. It is far easier for a for-profit tourist operation to be part of a freight line, although the rules are usually more strict when passengers come into play.